Cu2+ Removal in a Biosorption
Column by Immobilized Bacterial Biomass
of excess heavy metal ions from waste waters is essential due to their extreme toxicity
towards aquatic life and humans. Microorganisms are being increasingly studied for the
removal of heavy metal ions from aqueous solution. In this study, bacterial biomass, which
was isolated from soil beforehand, was identified as Bacillus subtilis. Then the
bacterial biomass was immobilized in agar and polyacrylamide gel. The immobilized
particles packed in a column were used to remove Cu2+. The effects of the
immobilization method, bed-length, flow rate and initial metal concentration on
performance of the Cu2+ removal by the fixed bed columns were systematically
investigated. The highest Cu2+ uptake efficiency 58.0% (Yi) was obtained by
Bacillus subtilis immobilized on agar, 10cm bed length, 180ml h-1 flow rate and
100mg l-1 initial Cu2+ concentration. This research showed that Bacillus
subtilis had a potential to be used in removal of Cu2+ from waste waters.
Freundlich constants were determined from the Freundlich adsorption isotherms.